Description
Azospirillum Medium w/o Agar (Twin Pack) PartA and B, Himedia
M1720
Azospirillum Medium w/o Agar (Twin Pack) is recommended for cultivation of Azospirillum species. This medium contains malic acid which serves as the prime carbon source. Azospirillum species grow well in presence of malic acid and are not overgrown by other nitrogen fixers. Necessary trace elements like ferrous sulphate, manganese sulphate, sodium molybdate supports growth of Azospirillum species.
แนะนำให้ใช้ Azospirillum Medium ที่ไม่มีวุ้น (แพ็คคู่) สำหรับการเพาะพันธุ์ Azospirillum ตัวกลางนี้มีกรดมาลิกซึ่งทำหน้าที่เป็นแหล่งคาร์บอนหลัก สายพันธุ์อะโซสไปริลลัมเจริญเติบโตได้ดีเมื่อมีกรดมาลิก และไม่เจริญเติบโตมากเกินไปโดยสารตรึงไนโตรเจนชนิดอื่น ธาตุที่จำเป็น เช่น เหล็กซัลเฟต แมงกานีสซัลเฟต โซเดียมโมลิบเดต ช่วยสนับสนุนการเจริญเติบโตของสายพันธุ์อะโซสไปริลลัม
Composition**
Ingredients Gms / Litre
Part A: –
Malic acid 5.000
Dipotassium phosphate 0.500
Ferrous sulphate 0.500
Manganese sulphate 0.010
Magnesium sulphate 0.200
Sodium chloride 0.100
Bromo thymol blue 0.002
Sodium molybdate 0.002
Calcium chloride 0.020
Part B: –
Potassium hydroxide 4.000
Final pH ( at 25°C) 6.8±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 6.33 grams in 950 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving
at 15 lbs pressure (121°C) for 15 minutes. Cool to room temperature and slowly add 50 ml of sterile Part B prepared by adding
1.30 grams -1.50 grams of Potassium hydroxide to 50 ml of sterile distilled water.
Principle And Interpretation
Azospirilla are generally gram-negative rods which are motile by means of a single flagellum. Azospirilla are also
capable of fixing nitrogen (1). The cells of Azospirillum remain in association with the roots and fix part of the atmospheric nitrogen. Azospirillum species may be isolated from rhizosphere biofilms associated with the roots of various grasses, cereals, and tuber plants. Their isolation is based on the fact that these organisms can grow in concentrations of nitrogen too low to support growth of most microorganisms. Azospirillum cultures are useful for the cereals and cash crops viz. wheat, paddy, bajra, jowar, maize, mustard, cotton, cumin, banana, sugarcane, tobacco, castor, vegetables etc., as well as horticultural crops.Both in greenhouse and in field trials, several strains of Azospirillum have been shown to exert beneficial effects on plant growth and crop yields, under various soil and climatic conditions, and are thus qualified as Plant Growth-Promoting
Rhizobacteria (PGPR) (2).
This medium contains malic acid which serves as the prime carbon source. Azospirillum species grow well in presence of
malic acid and are not overgrown by other nitrogen fixers. Dipotassium phosphate provides buffering effect. Necessary trace elements like ferrous sulphate, manganese sulphate, sodium molybdate supports growth of Azospirillum species. Divalent salts like calcium and magnesium helps metabolism of cells. Sodium chloride helps to maintain osmotic balance of cell.
Quality Control
Appearance
Part A : Cream to yellow homogeneous free flowing powder Part B :White to cream pellets
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent solution
Reaction
Reaction of 0.63% w/v aqueous solution at 25°C. pH : 6.8±0.2
pH
HiMedia Laboratories Technical Data
6.60-7.00
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for upto 8 days .
Cultural Response
Organism Growth
Cultural Response
Azospirillium brasilense
ATCC 29710
luxuriant
